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ISSN : 2287-5824(Print)
ISSN : 2287-5832(Online)
Journal of The Korean Society of Grassland and Forage Science Vol.36 No.3 pp.243-247

Arsenic-Induced Differentially Expressed Genes Identified in Medicago sativa L. roots

Md Atikur Rahman, Sang-Hoon Lee, Ki-Yong Kim, Hyung Soo Park, Tae Young Hwang, Gi Jun Choi, Ki-Won Lee*
Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea
*Corresponding author : Ki-Won Lee, National Institute of Animal Science, Cheonan 31000, Korea. Tel: +82-41-580-6757, E-mail:


Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.